| 產品名稱 |
31BG |
| 商品貨號 |
B221889 |
| Designations |
31BG |
| GenBank Number |
M86699
|
| Species |
Homo sapiens, human |
| Depositors |
GB Mills, R Schmandt |
| Vector |
Construct size (kb): 5.510000228881836 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TTK
Genomic copy number: unique
Gene name: TTK kinase (97 kDa)
Contains complete coding sequence?: N
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 2.55
Cross references: DNA Seq. Acc.: M86699 Insert lengths(kb): 2.549999952316284 Gene product: TTK kinase (97 kDa) [TTK] |
| Insert Size (kb) |
2.550 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglI--5.4; EcoRI--2.9, 1.7, 0.46, 0.36; EcoRV--5.4; SstI--2.9, 2.5; XbaI--5.4. The insert contains the following restriction sites (approximate kb from the 5' end): BglII--2.39; EcoRI--0.4, 0.84, 2.46; EcoRV--2.42; XbaI--0.80. Corresponds to nt 977-3528 of the published sequence, and includes most of the coding sequence (nt 978-3551). |
| References |
Mills GB, et al. Expression of TTK, a novel human protein kinase, is associated with cell proliferation. J. Biol. Chem. 267: 16000-16006, 1992. PubMed: 1639825
Schmandt R, et al. IL-2-induced expression of TTK, a serine, threonine, tyrosine kinase, correlates with cell cycle progression. J. Immunol. 152: 96-105, 1994. PubMed: 8254211
|
