| Vector |
Construct size (kb): 4.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MDU1
Genomic copy number: unique
Gene name: antigen identified by monoclonal antibodies 4F2, TRA1.10,
TROP4, and T43
Contains complete coding sequence?: N
Chromosome: 11; Localization: 11 q12.1-q13.1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.25
Cross references: DNA Seq. Acc.: J02769; EST: EST05215
Nucleotides 1-316 of the insert correspond to
nucleotides 1802-1486 of J02769 with 97% identity.
DNA Seq. Acc.: T07326; EST: EST05215; Primer: M13-21; THC
id: THC122463 Insert lengths(kb): 1.25 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43 [MDU1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.25. Based on the alignment with GenBank accession J02769 (1822 nt, CDS 54-1643) and the size of the insert, this clone appears to contain part of the coding sequence and the 3' untranslated region. |
