| Growth Conditions |
Temperature: 25.0°C Duration: grown with bacteria Protocol: ATCCNO: 30916 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in a T-25 tissue culture flask containing 10 ml ATCC medium 802 inoculated 24 hours previously with Enterobacter aerogenes ATCC 13048. Transfer any residual liquid from the vial to the flask. Place at 25C. Excystment should occur within a few days. |
| Subcultivation |
Protocol: ATCCNO: 30916 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in a T-25 tissue culture flask containing 10 ml ATCC medium 802 inoculated 24 hours previously with Enterobacter aerogenes ATCC 13048. Transfer any residual liquid from the vial to the flask. Place at 25C. Excystment should occur within a few days. |
| References |
Novotny RT. Colpoda spiralis sp., n., a colpodid ciliate found inhabiting treeholes (Colpodida, Ciliophora). J. Protozool. 24: 364-369, 1977.
Wickham SA, Lynn DH. Relations between growth rate, cell size, and DNA content in Colpodean Ciliates (Ciliophora: Colpodea). Eur. J. Protistol. 25: 345-352, 1990.
Nanney DL, et al. Comparison of sequence differences in a variable 23S rRNA domain among sets of cryptic species of ciliated protozoa. J. Eukaryot. Microbiol. 45: 91-100, 1998. PubMed: 9495037
type strain of Colpoda spiralis
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