Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--2.7, 1.5; BamHI/XhoI--3.4, 0.8; BglI--3.0, 1.2. Expression of ble (bleomycin resistance) is regulated by the promoter and terminator regions of the am gene (amination, glutamate dehydrogenase) from N. crassa. Shuttle vector conferring bleomycin and phleomycin resistance. The order of the major features in this plasmid is: pUC8 - EcoRI - BamHI - am promoter - ble - XhoI - am 3' region - HindIII - pUC8. |
