ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
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ATCC VR-2153
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
In Escherichia coli containing the plasmid
Comments
Also available as the vaccinia recombinant, vTF7-3, ATCC® VR-2153™.
When this plasmid is transfected into vaccinia-virus infected cells, T7 RNA polymerase activity is expressed.
The vector pGS53 includes a P7.5 promoter with early and late regulatory signals upstream of BamHI and SmaI cloning sites. These are flanked by vaccinia TK sequences from the Wyeth strain to direct recombination with vaccinia virus.
References
Fuerst TR, et al. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83: 8122-8126, 1986. PubMed: 3095828
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