| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--7.2, 2.6, 1.6; AvaI--3.2, 2.7, 1.9, 1.6, 1.2, 0.7; PstI--5.75, 4.8, 0.85; HindIII--6.8, 4.6; BamHI--7.0, 4.4. The insert contains the following restriction sites (approximate kb from the 5' BamHI site): EcoRI--2.0, 3.6 (exon 2); HindIII--6.6 (exon 7); PstI--4.6, 5.5. Nuclear proteins from tissues expression the gene bind specifically to two regions of intron 3. The sequence of the intron is available in GenBank J04465. A BanII fragment (nt -639 to +1395, or nt 2.0 to nt 4.03 relative to the 5' BamHI site), has been demonstrated to contain sequences recognized by developmentally regulated male transacting factors. Transcriptional control is positive. This fragment has been used to produce transgenic mice which express the gene at high levels in the preputial gland of males and at somewhat lower levels in livers of males. |
| References |
Sarkar P, Feigelson P. Developmentally regulated male-specific transfactor(s) enable in vitro transcription of a cloned alpha 2u-globulin gene. Mol. Endocrinol. 3: 342-348, 1989. PubMed: 2469008
Choy HA, et al. Nuclear factors from expressing tissues interact in vitro with a rat alpha-2u globulin gene intron. J. Biol. Chem. 264: 1754-1759, 1989. PubMed: 2463987
Dolan KP, et al. The structure and expression of very closely related members of the alpha 2u globulin gene family. J. Biol. Chem. 257: 13527-13534, 1982. PubMed: 6183262
Soares V da C, et al. Tissue-specific and hormonally regulated expression of a rat alpha 2u globulin gene in transgenic mice. Mol. Cell. Biol. 7: 3749-3758, 1987. PubMed: 2446121
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