| Vector |
Construct size (kb): 12.39999961853027 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacI BstXI SacII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ITK
Genomic copy number: unique
Gene name: T-cell-specific tyrosine kinase; homolog of mouse T-cell
itk/tsk tyrosine kinase
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q31-q32
Tissue: thymus
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 9.4
Cross references: DNA Seq. Acc.: L10717 Insert lengths(kb): 9.399999618530273 Tissue: thymus Gene product: T-cell-specific tyrosine kinase; homolog of mouse T-cell itk/tsk tyrosine kinase [ITK] |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.5, 3.6, 2.5; EcoRI--4.8, 3.1, 2.5, 2.0; PstI--9.2, 2.8, 0.56; XbaI--5.9, 3.8, 1.8, 0.7; HindIII--4.5, 4.3, 2.5, 1.1. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--5.92; BglI--2.13; BglII--4.67; EcoRI--1.95; EcoRV--4.48; HindIII--1.32, 2.43; KpnI--1.90; PstI--2.83, 3.39; SstI--2.19, 3.11; XbaI--5.26, 5.95. Contains the complete coding sequence (nt 2024-3886). A 2.8 kb PstI fragment from the unique and untranslated 5' domain has been used as a probe. |
| References |
Gibson S, et al. Identification, cloning, and characterization of a novel human T-cell-specific tyrosine kinase located at the hematopoietin complex on chromosome 5q. Blood 82: 1561-1572, 1993. PubMed: 8364206
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