| 產品名稱 |
pAIM-NEO |
| 商品貨號 |
B224877 |
| Designations |
pAIM-NEO |
| GenBank Number |
Z22576
|
| Species |
Homo sapiens, human |
| Depositors |
F Sanchez-Madrid |
| Applications |
in another host, produces protein CD69 antigen (p60, early T-cell activation antigen) |
| Vector |
Construct size (kb): 7.699999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDNA I/Neo
Intact vector size: 7.000
Type of vector: phagemid
Vector end: BstXI
Vector end: BstXI
Cloning sites: HindIII BamHI BstXI EcoRV NotI XhoI NsiI XbaI
Polylinker sites: HindIII BamHI BstXI EcoRV BstXI NotI XhoI NsiI XbaI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): supF, G418R
promoter: CMV
replicon: SV40, polyoma, pMB1, M13
terminator: SV40 polyadenylation
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD69
Genomic copy number: unique
Gene name: CD69 antigen (p60, early T-cell activation antigen)
Contains complete coding sequence?: Y
Chromosome: 12; Localization: 12 p13-p12
Tissue: lymphocyte, activated
Type of DNA: cDNA
Insert end: BstXI
Insert end: BstXI
Insert size (kb): 0.693
Cross references: DNA Seq. Acc.: Z22576
Nucleotides 1-694 of the insert correspond to
nucleotides 12-705 of Z22576. Insert lengths(kb): 0.6930000185966492 Tissue: lymphocyte, activated Gene product: CD69 antigen (p60, early T-cell activation antigen) [CD69] Target Gene: CD69 antigen (p60, early T-cell activation antigen) |
| Insert Size (kb) |
0.693 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII/NotI--7.7; BstXI--7.0, 0.7; HindIII--7.7; NcoI--2.45, 2.1, 1.55, 0.85 (doublet); SstI(SacI)--7.7. The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.07; NcoI--0.52; BstXI--0.52. Contains the complete coding sequence (nt 82-681 of the sequence record). Expresses the protein from the CMV promoter in mammalian cells. |
| References |
Lopez-Cabrera M, et al. Molecular cloning, expression, and chromosomal localization of the human earliest lymphocyte activation antigen AIM/CD69, a new member of the C-type animal lectin superfamily of signal-transmitting receptors. J. Exp. Med. 178: 537-547, 1993. PubMed: 8340758
Francisco Sanchez-Madrid, personal communication
|
