| 產(chǎn)品名稱 |
pBSGPX2 |
| 商品貨號(hào) |
B225949 |
| Designations |
pBSGPX2 |
| GenBank Number |
X68314
|
| Species |
Homo sapiens, human |
| Depositors |
F Chu |
| Vector |
Construct size (kb): 3.940000057220459 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacI BstXI SacII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GPX2
Other gene symbols: GSHPX-GI
Gene name: glutathione peroxidase 2 (gastrointestinal)
Contains complete coding sequence?: Y
Chromosome: 14; Localization: 14
Tissue: liver
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.971
Cross references: DNA Seq. Acc.: X68314
Nucleotides 1-971 of the insert correspond to
nucleotides 1-971 of X68314. Insert lengths(kb): 0.9710000157356262 Tissue: liver Gene product: glutathione peroxidase 2 (gastrointestinal) [GPX2] |
| Insert Size (kb) |
0.971 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.0; SphI--4.0; SmaI--3.1, 0.9; PstI--3.7, 0.3; BglII--4.0. The insert contains the following restriction sites (approximate kb from the 5' end): AvaI--0.20; BglII--0.64; NcoI--0.05; PstI--0.76; SmaI--0.20; SphI--0.50. |
| References |
Chu FF, et al. Expression, characterization, and tissue distribution of a new cellular Selenium-dependent glutathione peroxidase, GSHPx-GI. J. Biol. Chem. 268: 2571-2576, 1993. PubMed: 8428933
|
