| Vector |
Name of construct: AM6
Other name(s): pAM6
Construct size (kb): 7.5
Markers: ampR
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI, ClaI, HindIII, EcoRV, BamHI, SphI, SalI, XmaIII, NruI, BspMI, BsmI, StyI, AvaI, BalI, BspMII, PvuII, Tth111I, NdeI, AflIII, PpaI, PstI, PvuI, ScaI, SspI, AatII
Construction: pBR313
Host Range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross ref: DNA seq. Acc.: J01749
Transformant name: EC-AM6 |
| Comments |
The virus was isolated from plasma (subtype adw) from an HBsAg-positive donor. DNA was extracted from purified Dane particles and cloned into the pBR322 vector.
The complete viral genome was linearized with BamHI and inserted into pBR322. A BamHI digest cuts the insert into fragments of 1.85 kb and 1.35 kb. The 1.35 kb fragment contains coding sequences for HBsAg and most of region P. The 1.85 kb fragment encodes HBcAg and most of the X gene product. The 1350 bp BamHI fragment encodes both group and subtype-specific determinants of HBsAg. This AM6 clone is available as ATCC 39630, 40101, 45020 and 45020D. ATCC 40101 and 45020D are the purified plasmid DNAs and ATCC 39630 and 45020 are the plasmids in E. coli. Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.85, 1.35; EcoRI--7.5; BglII/PstI--5.2, 1.35, 0.96. |
