| Comments |
Restriction digests of the clone give the following sizes (kb): XbaI--5.5; BglI--2.3, 2.0, 0.71; BamHI--4.4, 0.94; EcoRV/SalI--3.85, 1.4; HindIII/SalI--3.8, 1.5; HindIII/XbaI--4.4, 0.65; HindIII/BglI--2.2, 1.1, 0.85, 0.72. The insert contains internal XbaI and BglI sites. Constructed from pCD-HT-5 by subcloning the 0.94 kb BamHI fragment into the BamHI site of pBR322. |
