| 產品名稱 |
human CAV cDNA |
| 商品貨號 |
B227606 |
| Designations |
human CAV cDNA |
| GenBank Number |
L19297
|
| Species |
Homo sapiens, human |
| Depositors |
WS Sly |
| Vector |
Construct size (kb): 4.079999923706055 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: XhoI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CA5
Genomic copy number: unique
Gene name: carbonic anhydrase V
Contains complete coding sequence?: Y
Chromosome: 16
Tissue: liver
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI linker
Insert 3' end: Modification: XhoI linker
Insert size (kb): 1.123
Cross references: DNA Seq. Acc.: L19297
Nucleotides 1-1123 of the insert correspond to
nucleotides 1-1123 of L19297. Insert lengths(kb): 1.123000025749207 Tissue: liver Gene product: carbonic anhydrase V [CA5] |
| Insert Size (kb) |
1.123 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: frozen |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.1; BamHI/XhoI--3.0, 1.1; BglI--1.9, 1.3, 0.9; EcoRI--3.6, 0.52; PstI--3.6, 0.4. The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.29; BglI--0.38; EcoRI--0.53; PstI--0.63. |
| References |
Nagao Y, et al. Human mitochondrial carbonic anhydrase: cDNA cloning, expression, subcellular localization, and mapping to chromosome 16. Proc. Natl. Acad. Sci. USA 90: 7623-7627, 1993. PubMed: 8356065
|
