| 產品名稱 |
clone 3.10 |
| 商品貨號 |
B227986 |
| Designations |
clone 3.10 |
| GenBank Number |
M59911
|
| Species |
Homo sapiens, human |
| Depositors |
Q Yuan, ME Hemler |
| Vector |
Construct size (kb): 6.730000019073486 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: XbaI closer to SacI
Vector end: XbaI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ITGA3
Gene name: integrin, alpha 3 (CD49C, alpha 3 subunit of VLA-3
receptor)
Contains complete coding sequence?: Y
Tissue: endothelium cells and HepG2 cell line
Type of DNA: cDNA
Insert end: Modification: XbaI linker
Insert end: Modification: SalI linker
Insert size (kb): 3.47
Cross references: DNA Seq. Acc.: M59911
Nucleotides 1-3470 of the insert correspond to
nucleotides 1-3470 of M59911. Insert lengths(kb): 3.47000002861023 Tissue: endothelium cells and HepG2 cell line Gene product: integrin, alpha 3 (CD49C, alpha 3 subunit of VLA-3 receptor)(integrin, alpha 3 subunit) [ITGA3] |
| Insert Size (kb) |
3.470 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): XbaI--3.5, 3.0; HindIII--6.5; EcoRI--6.5; BamHI--3.1, 2.9, 0.5; NdeI--6.5. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--2.39; EcoRI--2.47; BamHI--2.7, 3.32. Constructed from clones 3.410, 3.285 and 3.520. |
| References |
Takada Y, et al. Molecular cloning and expression of the cDNA for alpha 3 subunit of human alpha 3 beta 1 (VLA-3), an integrin receptor for fibronectin, laminin, and collagen. J. Cell Biol. 115: 257-266, 1991. PubMed: 1655803
|
