| Insert |
DNA: cDNA Insert lengths(kb): 2.700000047683716 Tissue: fibroblast Gene product: procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase; thyroid hormone binding protein p55) [P4HB] |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI/BamHI--2.4, 1.6, 0.82, o.6; BamHI--3.25, 1.9, 0.41; HindIII--3.4, 2.7; XhoI--3.1, 1.2, 1.1, 0.6; PstI--2.3, 1.6, 1.35, 0.6. Includes 5' untranslated sequences, the complete coding sequence, and a 3' untranslated sequence. A full length cDNA clone. The insert includes the following restriction sites (nt from 5' end): PstI--509, 2081; AvaI--133, 979, 1525, 1617, 1637, 2327; BamHI--1801, 2139; HindIII--2022. This protein shows homologies to the rat protein disulfide isomerase and the beta subunit of human prolyl-4-hydroxylase. |
| References |
Popescu NC, et al. Chromosomal localization of the gene for human thyroid hormone-binding protein. Am. J. Hum. Genet. 42: 560-564, 1988. PubMed: 2831713
Cheng SY, et al. The nucleotide sequence of a human cellular thyroid hormone binding protein present in endoplasmic reticulum. J. Biol. Chem. 262: 11221-11227, 1987. PubMed: 3611107
Sheue-Yann Cheng, personal communication
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