| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--13.5, 6.2, 5.4 (doublet), 3.9, 2.4, 2.0, 1.2, 0.7; BamHI--18.0 (doublet), 4.5, 3.8; HindIII--23.0, 6.0, 3.9, 2.5, 2.2, 1.9, 1.7, 1.1, 1.0; PstI--13.0, 5.9, 4.0 (doublet), 2.9, 2.5, 2.2, 2.0, 1.8, 1.5, 1.3, 1.2 + smaller. The insert can be excised from the vector with EcoRI, resulting in a 5.5 kb EcoRI vector fragment. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: BamHI, BglII, HindIII, HincII, RsaI, TaqI (panel of 5 individuals). |
| References |
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
Keith TP, et al. Genetic linkage map of 43 DNA markers on human chromosome 16. Proc. Natl. Acad. Sci. USA 87: 5754-5758, 1990. PubMed: 2377614
Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474
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