| Vector |
Construct size (kb): 6.329999923706055 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCRII
Intact vector size: 3.900
Type of vector: phagemid
Vector end: TA site
Vector end: TA site
Cloning sites: TA cloning between EcoRI sites
Polylinker sites: NsiI HindIII KpnI SacI BamHI SpeI BstXI EcoRI EcoRI EcoRV
BstXI NotI AvaI XmaIII XhoI NsiI XbaI ApaI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR, kanR
promoter: lac, SP6, T7
replicon: pMB1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RRM1
Genomic copy number: unique
Gene name: ribonucleotide reductase M1 polypeptide
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11 p15.5-p15.4
Type of DNA: cDNA
Insert 5' end: Modification: SacI site
Insert 3' end: Modification: PstI site
Insert size (kb): 2.379
Cross references: DNA Seq. Acc.: X59617
Nucleotides ~1~2379 of the insert correspond to
nucleotides 182-2560 of X59617. Insert lengths(kb): 2.378999948501587 Gene product: ribonucleotide reductase M1 polypeptide(ribonucleoside diphosphate reductase, M1 subunit) [RRM1] Alleles: A2, A2, A1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 1.7; SacI--4.0, 2.3; PstI--2.7, 2.4, 1.2; HindIII--3.7, 2.3; PstI/SacI--2.7, 2.4, 1.2. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.07; EcoRI--0.39, 0.93, 1.69; BamHI--0.53, 2.37. Constructed by PCR using primers generating a SacI site just upstream of the initation codon (5' GCTCGAGCTCATG 3') and a PstI site just after the termination codon (5' TGAG CTGCAGCTGC 3'). |
| References |
Pavloff N, et al. Sequence analysis of the large and small subunits of human ribonucleotide reductase. DNA Seq. 2: 227-234, 1992. PubMed: 1627826
Yun Yen, personal communication
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