To convert bacteriophage lambda clones to plasmid via the cre-lox recombination system, use of BNN132 (ATCC 47059) is recommended.
Vector
DNA: genomic
Insert size range: 4 - 7 kb
Media
ATCC® Medium 1592: SM buffer
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010
Ramer SW, et al. Dominant genetics using a yeast genomic library under the control of a strong inducible promoter. Proc. Natl. Acad. Sci. USA 89: 11589-11593, 1992. PubMed: 1454852
