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Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human
Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human
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貨期:
編號(hào):B230470
品牌:Mingzhoubio

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產(chǎn)品名稱(chēng) Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human
商品貨號(hào) B230470
Organism Homo sapiens, human
Tissue Blood
Cell Type Mononuclear
Morphology spherical; variable after culturing
Growth Properties suspension; variable after culturing
Biosafety Level 1

 [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens. 

ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.] 

Human Material Precaution 
All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.


Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age lot specific
Gender lot specific
Ethnicity lot specific
Applications Applications for use include the study of immunology, infection, cancer, hematology, and t-cell suppression assay.
Product Format frozen 1 mL
Storage Conditions -130°C
Images
Comments Peripheral blood mononuclear cells (PBMC) have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining peripheral blood mononuclear cells in culture in the absence of application-specific growth factors. 

Peripheral blood mononuclear cells are a heterogeneous population of blood cells with a single round nucleus and include macrophages, dendritic cells, monocytes and lymphocytes.

Complete Growth Medium Peripheral blood mononuclear cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining peripheral blood mononuclear cells in culture in the absence of application-specific growth factors.

Note: Application specific medium, cytokines and other factors

Subculturing N/A
Volume 1 mL
Cells per Vial One vial contains a minimum of 2.5 x 107 viable cells.
Viral Testing
Hepatitis B: Negative
Hepatitis C: Negative
HIV(I/II): Negative
HTLV(I/II): Negative
WNV: Negative
Trypanasome: Negative
Viability ≥ 70% when thawed from cryopreservation
Population Doubling Capacity N/A
C of A
Certificate of Analysis
Certificate of Analysis
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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