| 產品名稱 |
pPLc28-HIIF52 [HIIF-E] |
| 商品貨號 |
B230556 |
| Designations |
pPLc28-HIIF52 [HIIF-E] |
| Species |
Homo sapiens, human |
| Depositors |
Biogen N.V. |
| Applications |
produces protein interferon gamma |
| Vector |
Construct size (kb): 3.700000047683716 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pPLc28
Intact vector size: 2.720
Type of vector: plasmid
Cloning sites: EcoRI BamHI HindIII
Polylinker sites: EcoRI BamHI HindIII
Construction: pBR322, lambda
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: lambdaPL
replicon: pMB1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IFNG
Genomic copy number: unique
Gene name: interferon, gamma
Contains complete coding sequence?: U
Chromosome: 12; Localization: 12 q24.1
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.1
Cross references: Insert lengths(kb): 1.100000023841858 Gene product: interferon gamma [IFNG] Target Gene: interferon gamma |
| Insert Size (kb) |
1.100 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): ClaI/HindIII--2.9, 1.0; BamHI--3.9; EcoRI--uncut; PstI--3.9; BglI--2.1, 1.8. Absence of a transcription terminator 3' to the insert may cause instability of the plasmid after induction, due to interference with plasmid replication. When induced at 42C, interferon gamma (insoluble) can be produced to a level up to 14.7 percent of the total cellular protein. Consists of pPLc28 with the lambda PL promoter, a manipulated ribosome binding site from the E. coli trp attenuator, an engineered ATGTGTTAC sequence, and a sequence corresponding to nt 156-1170 of the sequence record. |
| References |
Simons G, et al. High-level expression of human interferon gamma in Escherichia coli under control of the pL promoter of bacteriophage lambda. Gene 28: 55-64, 1984. PubMed: 6234201
Fiers WC, Allet B. DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields. US Patent 5,004,689 dated Apr 2 1991
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
