| Vector |
Construct size (kb): 5.099999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: XhoI
Vector end: XhoI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDKN1A
Other gene symbols: CIP1; WAF1*
Gene name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p21.2
Tissue: B lymphocyte (EBV-transformed)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: L25610 Insert lengths(kb): 2.099999904632568 Tissue: B lymphocyte (EBV transformed) Gene product: cyclin-dependent kinase inhibitor 1A (p21, Cip1)( melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein)) [CIP1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.2, 0.66, 0.58, 0.50, 0.34, 0.22; EcoRI--3.8, 1.2; KpnI--3.8, 0.92, 0.31; PvuII--2.8, 1.2, 0.92, 0.4; XhoI--3.0, 2.1. The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.14, 0.61, 1.25, 1.8; KpnI--0.92; EcoRI--1.0; XhoI--2.0. Contains the complete coding sequence (nt 79-573 of the sequence record). |
