Restriction digests of the clone give the following sizes (kb): XhoI--30.0, 18.0; SalI--20.0, 14.0, 13.0. Transcription initiated from the GAL1 promoter in yeast hosts can be terminated at the CYC1 terminators. The GAL1 promoter lacks a translational start signal. There is also some expression on glucose. Vector for the construction of cDNA libraries permitting regulated expression in yeast and simple conversion to plasmid via the cre-lox system. The plasmid derivative (pTRP, 8.0 kb) is a YE-type shuttle expression vector. |
