This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level
1
Vector Information
Size (kb): 4.4000000953674320
Vector: pSVI6B5 (plasmid)
Promoters: Promoter SV40 early
Construction: pUC118, SV40
Marker(s):ampR
Construct size (kb): 4.400000095367432
Features: marker(s): ampR promoter: SV40 early replicon: pMB1, SV40
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4; HindIII--4.4; BamHI--4.4.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Castellino FJ, Higgins DL. DNA encoding human plasminogen modified at the cleavage site. US Patent 5,087,572 dated Feb 11 1992
