Replication of the virus has not been observed in amphibian or mammalian cell lines.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Rainbow trout (Salmo gairdneri), West Virginia, 1960
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
Brook trout 4 weeks of age become ill and die 1 to 4 weeks after feeding the virus. Antigenic for Salmo gairdneri, Salvelinus frontinalis, goats and rabbits. Replication of the virus has not been observed in amphibian or mammalian cell lines. This is a serious salmonid fish pathogen. It apparently originated in North America but has since been introduced to Europe and Japan. Stable at -20C and in 50% glycerol at 4C.
Effect on Host
Yes, in vitro effects: cytopathic effect, refractile rounding
CPE; refractile rounding
Recommended Host
Recommended Host:? RTG-2 (ATCC? CCL-55TM)
Growth Conditions
Temperature: 18.0°C
Duration: 1 to 2 days; For best results cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent).
Name of Depositor
KE Wolf, MC Quimby
Special Collection
ATCC
Source
Rainbow trout (Salmo gairdneri), West Virginia, 1960
Year of Origin
1960
References
Wolf K, Quimby MC, Bradford AD. Egg-associated transmission of IPN virus of trouts. Virology 21: 317-321, 1963.
Snieszko SF, et al. Virus nature of infectious pancreatic necrosis in trout. Proc. Soc. Exp. Biol. Med. 104: 105-108, 1960. PubMed: 13845654
can be grown on other fish cell lines; salmonid fishes (Salvelinus fontinalis, Salmo gairdneri, S. trutta, S. clarki, Salvelinus namaycush, Oncorhynchus kisutch, Salmo salar)
