| 產(chǎn)品名稱 |
Hepatitis A virus |
| 商品貨號 |
B232201 |
| Classification |
Picornaviridae, Hepatovirus |
| Agent |
Hepatitis A virus |
| Strain |
HM 175 (clone 1) |
| Applications |
Enteric Research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Effect on Host |
No CPE expected. CPE at high titer, cell rounding, sloughing, degeneration |
| Recommended Host |
FRhK4 (ATCC® CRL-1688™) |
| Growth Conditions |
Temperature: 34.5°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 34.5°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 14-28 days |
| Name of Depositor |
RH Purcell, National Institute of Allergy and Infectious Diseases; ATCC |
| Special Collection |
NCRR Contract |
| Source |
Human stool specimens from an outbreak of the virus, Australia. |
| References |
Daemer RJ, et al. Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage. Infect. Immun. 32: 383-393, 1981. PubMed: 6260685
Feinstone SM, et al. Isolation of hepatitis A virus strain HM-175. US Patent 4,532,215 dated Jul 30 1985
|
