| Vector |
Construct size (kb): 4.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: BCL1
Genomic copy number: unique
Gene name: B cell CLL/lymphoma 1
Contains complete coding sequence?: N
Chromosome: 11; Localization: 11 q13.3
Genome: Homo sapiens
Gene symbol: CCND1
Other gene symbols: PRAD1; D11S287E
Genomic copy number: unique
Gene name: cyclin D1 (parathyroid adenomatosis 1)
Contains complete coding sequence?: N
Chromosome: 11; Localization: 11 q13
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: M73554; EST: EST06912
Nucleotides 1-310 of the insert correspond to
nucleotides 2608-2917 of M73554 with 97% identity.
DNA Seq. Acc.: T09020; EST: EST06912; Primer: M13 Reverse;
THC id: THC104466 Insert lengths(kb): 1.799999952316284 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: cyclin D1 (parathyroid adenomatosis 1) [BCL1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.5; HindIII/NotI--3.0, 1.8. Based on the aligment with GenBank accession M73554 (4221 nt, CDS 142-1029), this clone appears to contain 3' untranslated sequence only. |
| References |
Temperani P, et al. Occurence of a novel t(11;19)(q13;q13.3) in complete remission of acute promyelocytic leukemia. Cancer Genet. Cytogenet. 101: 35-38, 1998. PubMed: 9460497
Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585
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