| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.8, 4.8, 3.9, 3.3, 2.7, 2.4, 1.8, 1.5 (triplet) and smaller bands. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
