-20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Introduction
Cell Line Description:
Epithelial cells from a histologically normal adult human prostate were isolated and subsequently transfected with a plasmid carrying one copy of the human papillomavirus 18 (HPV-18) genome to establish the RWPE-1 (ATCC CRL-11609) cell line.
Cells from the RWPE-1 cell line were further transformed by Ki-ras using the Kirsten murine sarcoma virus (Ki-MSV) to establish the RWPE-2 cell line (ATCC CRL-11610). RWPE-2 cells form colonies in agar and tumors in nude mice.
See www.atcc.org for more information
Quality Control Specifications
> 100 kb in size by agarose gel electrophoresis
No RNA detected in the agarose gel
A260/A280 ratio ≥ 1.8
Tested and verified for PCR amplification and restriction digestion.
