| Vector |
Construct size (kb): 4.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS-
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D14S46E
Other gene symbols: NRL; D14S46
Gene name: DNA Segment, single copy, expressed probes
Contains complete coding sequence?: Y
Tissue: retina, adult
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI
Insert 3' end: Modification: HindIII
Insert size (kb): 1.204
Cross references: DNA Seq. Acc.: M81840
Nucleotides 1-1204 of the insert correspond to
nucleotides 24-1227 of M81840. Insert lengths(kb): 1.203999996185303 Tissue: retina, adult Gene product: DNA Segment, single copy, expressed probes [NRL] Target Gene: DNA Segment, single copy, expressed probes |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 1.0, 0.2, EcoRI/KpnI--3.0, 1.2; HindIII--4.0, 0.2; SacI--3.0, 1.2; XhoI--3.7, 0.5. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.96; NcoI--0.005, 0.135, 0.39, 0.44; PstI--0.34, 0.40, 0.65, 1.075; PvuII--0.35, 0.49, 0.55, 0.70; SacI--1.07; XhoI--0.78. In Northern analyses, the insert detects transcripts in retinal tissue of the following species: baboon (1.3 kb); bovine, mouse, and rat (2.0 kb, major); frog (4 kb, 2kb, both faint). |
