| 產品名稱 |
HFBBA57 |
| 商品貨號 |
B233375 |
| Designations |
HFBBA57 |
| GenBank Number |
M78296
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 4.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D13S182E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Chromosome: 13; Localization: 13
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.3
Cross references: DNA Seq. Acc.: M78296; EST: EST00444; Primer: M13 Forward Insert lengths(kb): 1.299999952316284 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: DNA Segment, single copy, expressed [D13S182E] |
| Insert Size (kb) |
1.300 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8, 0.5; PstI--4.3; HindIII--3.4, 0.9. The insert contains multiple EcoRI fragments (kb): 0.75, 0.5. It is important to note these may have been derived from different cDNAs. This EST has also been mapped to chromosome 8. EST00444 matches GenBank accession CPVATI with 93.90% identity over the sequence range: 322-241. |
| References |
Hawthorn LA, Cowell JK. Regional assignment of EST sequences on human chromosome 13. Cytogenet. Cell Genet. 72: 72-77, 1996. PubMed: 8565640
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
Polymeropoulos MH, et al. Chromosomal distribution of 320 genes from a brain cDNA library. Nat. Genet. 4: 381-386, 1993. PubMed: 8401586
J C Venter, personal communication
|
