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Shipping Information
Distributed: freeze-dried
Comments
Consists of 5 SfiI fragments, 3 internal ones (70, 110, and 300 kb) and the YAC ends (50 and 70 kb).
The smallest fragment found to contain all the exons for GART is a 45 kb BssHII fragment.
The GART gene is toward the acentric end of the clone and the IFNGT1 gene is within 150 kb of the centric end.
When transferred into CHO cells, the clone complements the purine requirement of CHO Ade-C cells (lacking PRGS/GARS) and Ade-CG cells (lacking PRGS/GARS and PAIS/AIRS).
Both ends of the insert map to chromosome 21, suggesting a non-chimeric insert in this YAC.
References
Gnirke A, et al. Cloning and in vivo expression of the human GART gene using yeast artificial chromosomes. EMBO J. 10: 1629-1634, 1991. PubMed: 2050105
Gnirke A, Huxley C. Transfer of the human HPRT and GART genes from yeast to mammalian cells by microinjection of YAC DNA. Somatic Cell Mol. Genet. 17: 573-580, 1991. PubMed: 1767336
Cook JR, et al. Sublocalization of the human interferon-gamma receptor accessory factor gene and characterization of accessory factor activity by yeast artificial chromosome fragmentation. J. Biol. Chem. 269: 7013-7018, 1994. PubMed: 8120065
Soh J, et al. Identification of a yeast artificial chromosome clone encoding an accessory factor for the human interferon gamma receptor: evidence for multiple accessory factors. Proc. Natl. Acad. Sci. USA 90: 8737-8741, 1993. PubMed: 8378357
