| Insert |
DNA: genomic Insert lengths(kb): 40.0 Gene product: retinol-binding protein 3, interstitial(retinol-binding protein 3, interstitial (includes former D10S64, D10S65, D10S66)) [RBP3] Alleles: A2, B2, A1, A2, A2, A2, B2, B2, B2, A1, A1, A1, A1, A1, A1, A2, A2, A2, B1, B1, B2, C1, D1, D2, A1, A2, A3, C2, A1, A1, A1, A1, A1, A1, A1, A1, A1, A2, A2, A2, A2, A2, A2, A2, A2, A2, B1, B1, B1, B1, B1, B1, B2, B2, B2, C1, C1, C1, D1, D1, D1, D2, D2, D2, A1, A1, A1, A2, A2, A2, A3, A3, A3, C2, C2, C2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--12.0, 8.4, 6.0, 4.4, 2.15, 1.65, 1.25, 1.2, 0.87, 0.8; BamHI--11.0, 8.8, 7.8, 5.5, 2.6, 2.15, 1.9, 1.7; HindIII--9.4, 8.4, 6.6, 4.4, 3.7, 3.1, 2.2, 1.9, 1.6; PstI--3.5, 3.0, 2.5, 2.3, 2.2, 2.1 + smaller; XhoI--31.0, 7.8, 0.78. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: PstI. |
