| Vector |
Construct size (kb): 7.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TAX
Other gene symbols: TAG1
Genomic copy number: unique
Gene name: transiently-expressed axonal glycoprotein (axonin-1)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 q32
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 4.5
Cross references: DNA Seq. Acc.: X68274 Insert lengths(kb): 4.5 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: axonal glycoprotein TAG-1( axonal glycoprotein TAG-1, axonin-1 cell adhesion molecule, transiently-expressed axonal glycoprotein, transiently-expressed axonal glycoprotein (axonin-1)) [TAX] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 3.0, 0.6; HindIII/XbaI--4.5, 2.95; BamHI--4.3, 3.0, 0.2; PstI--3.15, 1.1 (doublet), 0.9, 0.6, 0.5; KpnI--3.3, 3.25, 1.0. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.26, 3.15; BglI--0.02, 1.15, 1.84, 2.26, 3.37, 3.86; EcoRI--0.57; KpnI--0.40, 1.38; PstI--0.57, 1.05, 2.12, 3.00, 3.16, 4.23, 4.25; PvuII--0.11, 0.44, 1.03, 1.91, 2.05, 2.60, 2.75, 3.32; SacI--2.35, 3.29, 3.55, 3.78; SmaI--0.36, 1.50, 2.46; XhoI--3.80. Contains the complete coding sequence, starting at the 5' end of the published sequence, with an additional T at position 2986, responsible for a frame shift and early stop codon. The GenBank record and reference show the corrected sequence. |
| References |
Hasler TH, et al. cDNA cloning, structural features, and eukaryotic expression of human TAG-1/axonin-1. Eur. J. Biochem. 211: 329-339, 1993. PubMed: 8425542
Kenwrick S, et al. Localization of the human TAX-1 gene to 1q32.1: a region implicated in microcephaly and Van der Woude syndrome. Hum. Mol. Genet. 2: 1461-1462, 1993. PubMed: 8242070
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