人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 1G8
▍最近瀏覽歷史
▍聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
1G8
1G8
規格:
貨期:
編號:B238827
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 1G8
商品貨號 B238827
Organism Ictalurus punctatus, channel catfish
Tissue peripheral blood
Cell Type lymphocyte B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
They are reported to secrete moderate levels of IgM in culture [PubMed: 8133033].
Storage Conditions liquid nitrogen vapor phase
Karyotype diploid
Derivation
1G8 (ATCC CRL-2756), developed in 1992, and 3B11 (ATCC CRL-2757), developed in 1994, are immortal B cell lines developed by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from two different normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. They are reported to secrete moderate levels of IgM in culture [PubMed: 8133033]. The 1G8 cells express p53 mRNA levels only two to three times higher than comparable levels found in freshly isolated peripheral blood [PubMed: 11687262]. The 1G8 cells express the heat shock protein hsp70 gene in significantly higher levels than comparable levels found in freshly isolated peripheral blood [PubMed: 11687262]. The 1G8 cells constitutively express telomerase [PubMed: 9914913].
Comments
1G8 (ATCC CRL-2756), developed in 1992, and 3B11 (ATCC CRL-2757), developed in 1994, are immortal B cell lines developed by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from two different normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. They are reported to secrete moderate levels of IgM in culture [PubMed: 8133033]. The 1G8 cells express p53 mRNA levels only two to three times higher than comparable levels found in freshly isolated peripheral blood [PubMed: 11687262]. The 1G8 cells express the heat shock protein hsp70 gene in significantly higher levels than comparable levels found in freshly isolated peripheral blood [PubMed: 11687262]. The 1G8 cells constitutively express telomerase [PubMed: 9914913].
Complete Growth Medium A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine, and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 87.5%; double distilled water, 10%; heat-inactivated Catfish serum, 2.5%

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml.
Interval: Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 27.0°C
Name of Depositor NW Miller
Year of Origin 1992
References

Miller NW, et al. Development and characterization of channel catfish long term B cell lines. J. Immunol. 152: 2180-2189, 1994. PubMed: 8133033

Wilson M, et al. A novel chimeric Ig heavy chain from a teleost fish shares similarities to IgD. Proc. Natl. Acad. Sci. USA 94: 4593-4597, 1997. PubMed: 9114035

Stuge TB, et al. Development and analysis of various clonal alloantigen-dependent cytotoxic cell lines from channel catfish. J. Immunol. 164: 2971-2977, 2000. PubMed: 10706684

Barker K, et al. Immortal and mortal clonal lymphocyte lines from channel catfish: comparison of telomere length, telomerase activity, tumor suppressor and heat shock protein expression. Dev. Comp. Immunol. 26: 45-51, 2002. PubMed: 11687262

Miller N, et al. Functional and molecular characterization of teleost leukocytes. Immunol. Rev. 166: 187-197, 1998. PubMed: 9914913

Shen L, et al. Channel catfish cytotoxic cells: a mini-review. Dev. Comp. Immunol. 26: 141-149, 2002. PubMed: 11696379

Miller NW, et al. Development of leukocyte cell lines from the channel catfish (Ictalurus punctatus). J. Tissue Culture Methods 16: 117-123, 1994.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板： 久久精品娱乐亚洲领先| 天堂а√中文最新版地址在线| 无遮挡午夜男女xx00动态| 欧美孕妇xxxx做受欧美88| 日日噜狠狠噜天天噜av| 国产日韩精品中文字无码| 久国产精品人妻aⅴ| 日产乱码一二三区别免费麻豆 | 久久久久人妻一区精品果冻| 国产成人精品一区二三区| 亚洲成熟女人毛毛耸耸多| 亚洲人成电影网站色迅雷| 亚洲中字慕日产2020| 亚洲色欲综合一区二区三区| 人妻另类 专区 欧美 制服| 亚洲精品无码人妻无码| 伊人久久精品无码麻豆一区| 99re8这里有精品热视频免费| 亚洲va无码专区国产乱码| 成人一在线视频日韩国产| 久久国产亚洲高清观看| 久久精品国产免费观看| 成人试看30分钟免费视频| 超清精品丝袜国产自在线拍| 无码丰满少妇2在线观看| √天堂中文www官网在线| 国产清纯在线一区二区www| 美国一区二区三区无码视频| 男女后式激烈动态图片| 日韩中文高清在线专区| 国产高清一区二区三区直播| 97高清国语自产拍| 97影院理论片手机在线观看| 小12萝裸体自慰出白浆| 免费做爰猛烈吃奶摸视频在线观看| 一本色道久久hezyo无码| 亚洲va久久久噜噜噜久久男同| 日产a一a区二区www| 日本japanese丰满少妇| 黑人猛挺进小莹的体内视频| 亚洲妇女自偷自偷图片|