人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > EBO-pSV2-neo
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
EBO-pSV2-neo
EBO-pSV2-neo
規格:
貨期:
編號:B239996
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 EBO-pSV2-neo
商品貨號 B239996
Designations EBO-pSV2-neo
Depositors RF Margolskee
Biosafety Level 1
Vector Information
Size (kb): 13.3599996566772500
Vector: EBO-pSV2-neo (plasmid)
Promoters: Promoter SV40 late
Construction: pSV2neo, p220-1 (EBV oriP, EBNA-1, hph)
Marker(s):G418R,ampR,hygR
Construct size (kb): 13.35999965667725
Features: marker(s): ampR, hygR, G418R
promoter: SV40 late
replicon: pMB1, EBV oriP
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--13.4; SfiI--7.5, 6.1; PstI--7.4, 2.3, 1.5, 1.0; SacI--13.4; BamHI--6.8, 5.4; HindIII--13.4; BglII--6.6.
pcD cDNA libraries can be converted into EBO-pcD libraries by ligating the 7.4 kb SfiI fragment of EBO-pSV2-neo (containing oriP, EBNA-1, hph) into SfiI-linearized, phosphatased, pcD DNA.
A selectable, stably replicating shuttle vector for expression of cDNAs in eukaryotic cells. Maintained episomally.
The vector contains EBV oriP and EBNA to ensure replication as episomes in human or simian cells, even if the cell line is not EBNA positive.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Margolskee RF, et al. Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells. Mol. Cell. Biol. 8: 2837-2847, 1988. PubMed: 2841588

Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549

Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831

Okayama H, Berg P. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3: 280-289, 1983. PubMed: 6300662

Mulligan RC, et al. Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature 277: 108-114, 1979. PubMed: 215915

Shipped freeze-dried
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板: 精品熟人妻一区二区三区四区不卡| 国产亚洲精品a在线观看下载| 狠狠躁18三区二区一区| 精品乱码一区二区三四区视频| 欧美尺码专线欧洲b1b1| 女性自慰网站免费观看w| 国产99视频精品免费视看6| 蜜桃久久精品成人无码av| 亚洲人成人无码网www国产| 午夜福利不卡在线视频| 久久不见久久见www电影免费| 亚洲色欧美色2019在线| 国产成人综合久久久久久| 2019亚洲午夜无码天堂| 精品免费国产一区二区三区四区介绍 | 2021国产精品午夜久久| 试看120分钟做受小视频| 夜夜躁狠狠躁日日躁孕妇| 国产午夜成人无码免费| 97人妻熟女成人免费视频色戒| 精品国产乱码久久久久久1区2区 | 亚洲人av高清无码| 欧美va免费高清在线观看| 日韩免费无砖专区2020狼| 无码国产精品一区二区免费模式| 国产内射合集颜射| 国产成人精品三级在线影院| 精选国产av精选一区二区三区| 国产午夜视频在线观看| 久艹视频免费看| 在线观看无码av网站永久免费| 精品少妇一区二区三区免费观| 国产精品无码无卡无需播放器| 国产一区二区三区自产| 免费观看性欧美大片无片| 色悠久久久久久久综合网伊人| 免费视频成人片在线观看| 国产精品视频免费一区二区| 久久丫精品国产| 2021久久精品国产99国产精品| 亚州久久久久区1区2少妇|