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pERT84-10
pERT84-10
規格:
貨期:
編號:B242745
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pERT84-10
商品貨號 B242745
Designations pERT84-10
Species Homo sapiens, human
Depositors LM Kunkel
Vector
Construct size (kb): 6.900000095367432
Insert
DNA: genomic
Insert lengths(kb): 2.5
Gene product: DNA Segment, single copy (within DMD gene) [DXS142]
Alleles: A1, A2
Insert Size (kb) 2.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 1.8, 0.8; EcoRI--6.9; PstI--6.9; BamHI--4.4, 2.5; SalI--6.9.
This clone is at the 5' most end of the DMD locus.
HindIII digestion yields 2 fragments from the insert: 1.8 kb and 0.7 kb. The larger fragment recognizes the two constant TaqI bands of 3.4 and 1.75 kb. The 0.7 kb HindIII fragment detects the TaqI RFLP.
The polymorphisms detected by this probe are contained within the DMD locus.
References

Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877

Bertelson CJ, et al. Localisation of Xp21 meiotic exchange points in Duchenne muscular dystrophy families. J. Med. Genet. 23: 531-537, 1986. PubMed: 2879924

Kunkel LM, et al. Specific cloning of DNA fragments absent from the DNA of a male patient with an X chromosome deletion. Proc. Natl. Acad. Sci. USA 82: 4778-4782, 1985. PubMed: 2991893

Louis M Kunkel, personal communication

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